ABSTRACT
The present attempt has been made to evaluate, and examine the levels of minerals in serum and liver in DEN induced hepatocellular carcinoma in wistar albino rats for possible chemopreventive effect. In hepatocellular carcinogenesis complications such as hepatic fibrosis and cirrhosis may lead to several abnormalities in mineral metabolism, hence attempt is made to evaluate on the level of minerals. Hepatic cancer was induced by single dose of intraperitoneal injections of DEN (200mg/kg body weight) followed by phenobarbital of 0.05% mixed with drinking water for 20 weeks. Concentration of calcium, magnesium, sodium and potassium were assessed in the serum and liver at the end of experimental period. Negative correlations were observed between liver function tests and serum mineral levels, except with albumin. Calcium, magnesium, potassium and sodium concentrations in the serum were decreased after the induction of hepatic cancer. The liver calcium content was increased after DEN treatment. No change occurred in liver sodium content. However, magnesium and potassium content was significantly reduced in the hepatic tissue. The results suggest that in DEN-induced hepato cellular carcinoma alteration of essential elements is noted. The low levels of albumin and the related ascites may be one of the major causes of the imbalance of mineral metabolism in hepatocellular carcinoma. Abbreviations: N-Nitrosodiethylamine (DEN), Hepatocellular carcinoma (HCC), Captain Srinivasa Murti Drug Research Institute for Ayurveda (CCRAS), Tamilnadu Veterinary and animal Science University (TANUVAS), Institutional Animal Ethics Committee (IAEC).